Effect of plant growth regulator and explant types on in vitro callus induction of Gynura procumbens (Lour.) Merr

Nurokhman, Amin and Faizah, Hanik and Sugiharto, Sugiharto and Utami, Edy Setiti Wida and Manuhara, Yosephine Sri Wulan (2019) Effect of plant growth regulator and explant types on in vitro callus induction of Gynura procumbens (Lour.) Merr. Research Journal of Biotechnology, 14 (9). pp. 102-107. ISSN 0973-6263; 2278-4535

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Abstract

Gynura procumbens (Lour.) Merr is a medicinal plant of Asteraceae. The plant is a potent compound as pharmaceutical raw material which can be increased through plant tissue culture. This study aimed to determine the effect of various combinations of plant growth regulator and explant types on induction callus. The explants used were leaf, stem node, stem internode and petiole while the plant growth regulator used were 0.1 mg/L 2,4-D and 0.1 mg/L BAP, 0.5 mg/L 2,4-D and 1.0 mg/L Kinetin, 0.5 mg/L NAA and 0.5 mg/L BAP, 5.0 mg/L 2,4 D and 0.5 mg/L BAP, 0.1 mg/L 2,4-D and 0.1 mg/L IAA. The explants were cultured in MS medium supplemented with 30% sucrose and 8% agar for 28 days. The results of this study indicated that the treatment of 0.5 mg/L NAA and 0.5 mg/L BAP on the petiole explants was the best combination of plant growth regulators to produce the highest callus fresh and dry weights (1478.1 mg and 40.0 mg respectively). Callus derived from leaf, petiole and internode explants was friable and compact in texture while node explant-derived callus was compact in texture.

Item Type: Article
Creators:
Creators
Email
NIDN
Nurokhman, Amin
UNSPECIFIED
UNSPECIFIED
Faizah, Hanik
hanikfaizah@uinsby.ac.id
8927000020
Sugiharto, Sugiharto
UNSPECIFIED
UNSPECIFIED
Utami, Edy Setiti Wida
UNSPECIFIED
UNSPECIFIED
Manuhara, Yosephine Sri Wulan
wulanmanuhara@gmail.com
UNSPECIFIED
Uncontrolled Keywords: Gynura procumbens; callus induction; plant growth regulator; explants type
Subjects: 06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060705 Plant Physiology
11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences > 111504 Pharmaceutical Sciences
Divisions: Karya Ilmiah > Artikel
Depositing User: desiana ekasari
Date Deposited: 30 Jul 2021 08:23
Last Modified: 17 Nov 2021 07:48
URI: http://repository.uinsby.ac.id/id/eprint/551

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