Influence of combination of sucrose concentration and immersion frequency on biomass and flavonoid production of Gynura procumbens (Lour.) Merr Callus culture in temporary immersion bioreactor

Nurokhman, Amin and Tahani, Nadia Anisa and Faizah, Hanik and Utami, Edy Setiti Wida and Manuhara, Yosephine Sri Wulan (2018) Influence of combination of sucrose concentration and immersion frequency on biomass and flavonoid production of Gynura procumbens (Lour.) Merr Callus culture in temporary immersion bioreactor. Scholars Academic Journal of Biosciences (SAJB), 6 (12). pp. 748-754. ISSN 2347-9515; 2321-6883

[thumbnail of Hanik Faizah_Influence of combination of sucrose concentration.pdf] Text
Hanik Faizah_Influence of combination of sucrose concentration.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial.

Download (589kB)

Abstract

The concentration of sucrose, in many cases could influence the production of biomass and secondary metabolite because sucrose is the main carbon source of metabolism, while liquid culture method has many advantages but has problems such as asphyxia and hyperhydricity because of long immersion. Temporary immersion bioreactor could solve problems by way of the immersion frequency. The study aimed to investigate the influence of combination sucrose concentration and immersion frequency on biomass and flavonoid production of Gynura procumbens in temporary immersion bioreactor. Callus was obtained from stem nodes and stem internodes that were grown in MS agar medium supplemented with 0.1 mg L-1 2.4-D (2.4 dichlorofenoxyaceticacid) and 0.1 mg L-1 IAA (indole acetic acid). In temporary immersion bioreactor (modification of RITA) calli were cultured in MS liquid medium supplemented with various concentration of sucrose (3%, 5%, and 7%) and immersion frequency of 15 min at 12 h interval and 5 min at 3 h interval. Cultures were maintained for 28 days. Results
showed that combination of sucrose 5% and immersion frequency of 15 min at 12 h interval produced the highest biomass, while the combination of sucrose 7% and immersion frequency of 15 min at 12 h interval produced the highest flavonoid

Item Type: Article
Creators:
Creators
Email
NIDN
Nurokhman, Amin
UNSPECIFIED
UNSPECIFIED
Tahani, Nadia Anisa
UNSPECIFIED
UNSPECIFIED
Faizah, Hanik
hanikfaizah@uinsby.ac.id
8927000020
Utami, Edy Setiti Wida
UNSPECIFIED
UNSPECIFIED
Manuhara, Yosephine Sri Wulan
UNSPECIFIED
UNSPECIFIED
Uncontrolled Keywords: Gynura procumbens; temporary immersion bioreactor; biomass; flavonoid; callus culture
Subjects: 06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060705 Plant Physiology
Divisions: Karya Ilmiah > Artikel
Depositing User: desiana ekasari
Date Deposited: 29 Jul 2021 15:49
Last Modified: 17 Nov 2021 08:43
URI: http://repository.uinsby.ac.id/id/eprint/535

Actions (login required)

View Item
View Item