Growth and accumulation of flavan-3-ol in camellia sinensis through callus culture and suspension culture method

Sutini, Sutini and Susilowati, Susilowati and Indra, M. Rasjad and Purwanto, Djoko Agus (2017) Growth and accumulation of flavan-3-ol in camellia sinensis through callus culture and suspension culture method. Berkala Penelitian Hayati, 22 (1). pp. 27-31. ISSN 0852-6834; 2337-389X

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Abstract

This study was aimed to assess flavan-3-ol biomass in C. sinensis through callus cultures and suspension cultures derived from leaf explants. Callus initiation of both cultures were using Murashige and Skoog medium were enriched with plant growth regulators Naphthalene Acetic Acid 3.0 mg/L and kinetin 2.0 mg/L. The procedures in this study were: (1) callus initiation by cutting the leaves of C. sinensis shoots then planted on Murashige and Skoog medium that were enriched with plant growth regulators, (2) sub callus culture on fresh medium that enriched with the same growth regu-lators, (3) suspension culture initiation of liquid callus, (4) growth examination of callus and suspension cultures in week 12, (5) examination of qualitative-quantitative content of flavan-3-olin suspension cultures at week 4. The results show that suspension cultures contain biomass flavan-3-ol that increase in the same manner of the increase of callus age and weight.

Item Type: Article
Creators:
Creators
Email
NIDN
Sutini, Sutini
sutinimiskun@uinsby.ac.id
2003017703
Susilowati, Susilowati
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Indra, M. Rasjad
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Purwanto, Djoko Agus
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Uncontrolled Keywords: Biomassa flavan-3-ol; C. sinensis; Murashige and Skoog; naphthaleneacetic acid; kinetin
Subjects: 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060207 Population Ecology
07 AGRICULTURAL AND VETERINARY SCIENCES > 0701 Agriculture, Land and Farm Management > 070103 Agricultural Production Systems Simulation
11 MEDICAL AND HEALTH SCIENCES > 1101 Medical Biochemistry and Metabolomics > 110103 Medical Biochemistry - Inorganic Elements and Compounds
Divisions: Karya Ilmiah > Artikel
Depositing User: Samidah Nurmayuni
Date Deposited: 22 Jul 2021 14:41
Last Modified: 22 Jul 2021 14:41
URI: http://repository.uinsby.ac.id/id/eprint/460

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