Optimization of PCR protocols for ITS rDNA amplification of yeasts isolated from Apis mellifera Honeycomb

Arifa, Ayudya Fitri and Firdhausi, Nirmala Fitria and Hidayati, Irul and Rachmawati, Yuanita and Hadi, Moch. Irfan (2022) Optimization of PCR protocols for ITS rDNA amplification of yeasts isolated from Apis mellifera Honeycomb. Jurnal Biota, x (x). pp. 1-11. ISSN 2460-7746; 2528-262X

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Abstract

Yeast is a microorganism that can be found in honeycomb. Yeast identification is a process to find and identify new species. One of which is molecular identification of yeast with rDNA sequences in the ITS region. Before carrying out molecular identification, it is necessary to optimize yeast DNA amplification methods to obtain good DNA sequences that ease the yeast identification process. The purpose of this study was to discover the optimum PCR (Polymerase Chain Reaction)protocols for the identification of yeasts isolated from Apis mellifera honeycomb based on the ITS rDNA. This study used 3 PCR (Polymerase Chain Reaction) protocols, i.e., from Kanti et al. (2018), Ediningsari (2008), and Maulana (2011). This results study shows that the optimum PCR protocol was from Maulana (2011), which produced clear and whole DNA fragment luminescences.

Item Type: Article
Creators:
Creators
Email
NIDN
Arifa, Ayudya Fitri
-
-
Firdhausi, Nirmala Fitria
nirmala_firdhausi@yahoo.com
2025068501
Hidayati, Irul
irulhidayati.alfatawi@gmail.com
198102282014032001
Rachmawati, Yuanita
yuanitarhartono@gmail.com
2019088803
Hadi, Moch. Irfan
m_i_h@uinsby.ac.id
0824048601
Uncontrolled Keywords: Apis mellifera; PCR protocol yeast
Subjects: 11 MEDICAL AND HEALTH SCIENCES > 1101 Medical Biochemistry and Metabolomics > 110103 Medical Biochemistry - Inorganic Elements and Compounds
11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences > 110309 Infectious Diseases
Divisions: Fakultas Tarbiyah dan Keguruan > Pendidikan Ilmu Pengetahuan Alam
Depositing User: Samidah Nurmayuni
Date Deposited: 24 Mar 2022 08:30
Last Modified: 24 Mar 2022 08:30
URI: http://repository.uinsby.ac.id/id/eprint/2238

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